Proteins (Hericium erinaceum) identified by 2-DGE and N-terminal amino acid sequencing
The table lists indicated spot numbers, amino acid sequences, scores, accesion numbers, protein names, identity/similality, theoretical mass (kDa), theoretical pH, organism and function left to right.
Spot Number	Amino Acid Sequence	Score	Accession Number	Protein Name	Identity /Similarlity	Analytical MW	Theoretical pI	Organism	Function
1	qlttkvrvlrdkrin	51	Q6FJ50	Guanine nucleotide-binding protein subunit gamma*	53 / 73	10251.63	6.05	Fungi	Signal transduction
2	gwfhddsdeaqaysqvndaphkael	118	Q4IID0	Hypothetical protein	67 / 83	11878.88	4.88	Fungi	Unclassified
3	anpggdyamn			No significant hit found
4	qftvvlkaan	44	P58724	Blue-light photoreceptor*	70 / 80	28816.77	4.73	Bacteria	Signal transduction
5	qgatsikalgfnipl	59	Q9LA06	Serine protease do-like htrA*	62 / 92	41647.97	5.17	Bacteria	Amino acid transport and metabolism
6	hgeaeefilg	49	Q31G56	Ribonuclease HII*	70 / 90	22808.57	6.30	Bacteria	Nucleotide transport and metabolism
7	rakvyvmnls	40	P61174	50S ribosomal protein L36*	75 / 100	4694.62	12.14	Bacteria	Translation, ribosomal structure and biogenesis
8	txgdpsaavgflray			No significant hit found
9	txgdpsaavgflraylef	57	Q9YAD8	Hypothetical protein APE2001	57 / 86	15980.57	5.40	Bacteria	Unclassified
10	txgdpsaavgflraylef	57	Q9YAD8	Hypothetical protein APE2001	57 / 86	15980.57	5.40	Bacteria	Unclassified
11	flekaqikvgekvslsetvk	57	Q88QM6	30S ribosomal protein S17*	56 / 75	10057.76	9.81	Bacteria	Translation, ribosomal structure and biogenesis
12				N-terminal amino acid blocked
13	axpasflekaqikvgekvsl	67	Q6H806	Putative ubiquitin fusion degradation protein*	50 / 94	35143.74	5.77	Plant	Posttranslational modification, protein turnover, chaperones
14	vektahaaarsflekaqikv	68	Q8U778	Transcriptional regulator, ROK family*	58 / 89	42235.10	5.97	Bacteria	Transcription
15	qpilfiaddgyafty	62	Q9AJ64	Antigenic heat-stable 120 kDa protein	64 / 79	108518.46	5.08	Bacteria	Unclassified
16				N-terminal amino acid blocked
17				N-terminal amino acid blocked
18				N-terminal amino acid blocked
19	mllysdiitddemfsdafpv	101	Q5K7S2	Translationally-controlled tumor protein homolog *	65 / 95	18763.25	4.55	Fungi	Signal transduction
20	mllysdiitddemfsda	79	Q5K7S2	Translationally-controlled tumor protein homolog	65 / 94	18763.25	4.55	Fungi	Signal transduction
21	vhtlpdlpyaydalepyfsr	123	Q9P4T6	Superoxide dismutase [Mn]*	90 / 100	22194.25	6.03	Fungi	Inorganic ion transport and metabolism
22	vhtlpdlpyayd	77	Q9P4T6	Superoxide dismutase [Mn]	80 / 90	22194.25	6.03	Fungi	Inorganic ion transport and metabolism
23	vlklhgsphstgttrvvvvl	84	Q1EBD9	Glutathione S-transferase*	75 / 85	23889.39	6.60	Fungi	Secondary metabolites biosynthesis, transport, and catabolism
24	snavkeglffta			No significant hit found
25				N-terminal amino acid blocked
26	tltggrgafgir	56	Q1CX38	ATP synthase epsilon chain*	78 / 89	14595.68	5.54	Bacteria	Energy production and conversion
27	aprkffvggnfkmnpstqae	97	P04828	Triosephosphate isomerase	71 / 94	27156.97	5.88	Fungi	Carbohydrate transport and metabolism
28				N-terminal amino acid blocked
29	rplaglyrga			No signifcant hit found
30	kplfgv			No signifcant hit found
31	tqgpivtgtsilalk	71	A1CE34	Proteasome component Pre4*	67 / 100	29023.61	5.67	Fungi	Posttranslational modification, protein turnover, chaperones
32	mlgng			No significant hit found
33	qglpganyit			No significant hit found
34	kvgges			No significant hit found
35				N-terminal amino acid blocked
36				N-terminal amino acid blocked
37	pesredsvylaklaeqaery	125	Q4G2I8	14-3-3 protein	100 / 100	28721.07	4.71	Fungi	Signal transduction
38				N-terminal amino acid blocked
39	sihpevllgq			No significant hit found
40	mrliirddktavgdy	73	Q4PDU5	hypothetical protein	73 / 86	32424.87	6.35	Fungi	Unclassified
41				N-terminal amino acid blocked
42				N-terminal amino acid blocked
43	nkynagkygl	58	A1FRZ0	Lytic transglycosylase*	80 / 90	35129.87	8.28	Bacteria	Cell envelope biogenesis, outer membrane
44				N-terminal amino acid blocked
45	tkvavlgagggigqplslll	113	P17505	Malate dehydrogenase*	95 / 100	35649.96	8.46	Fungi	Energy production and conversion
46	spryilatdq			No significant hit found
47	pkavvlgaaggigqp	75	P17505	Malate dehydrogenase	79 / 100	35649.96	8.46	Fungi	Energy production and conversion
48	skavvlgaaggigqp	75	P17505	Malate dehydrogenase	79 / 100	35649.96	8.46	Fungi	Energy production and conversion
49				N-terminal amino acid blocked
50				N-terminal amino acid blocked
51	dyqtakpaievgklk	62	P36498	Transport protein comB*	83 / 92	49601.49	5.64	Bacteria	Cell envelope biogenesis, outer membrane
52	paltvtklnt	45	P92133	Cathepsin B-like CP3 precursor*	83 / 100	32673.83	5.19	Insect	Amino acid transport and metabolism
53				N-terminal amino acid blocked
54	dillfkvlr	49	Q5VV43	Uncharacterized protein KIAA0319 precursor	89 / 100	117762.74	5.34	Human	Unclassified
55				N-terminal amino acid blocked
56				N-terminal amino acid blocked
57	vvkvgingfgrigri	97	Q8J1H3	Glyceraldehyde-3-phosphate dehydrogenase	100 / 100	36400.27	6.46	Fungi	Carbohydrate transport and metabolism
58				N-terminal amino acid blocked
59				N-terminal amino acid blocked
60				N-terminal amino acid blocked
61				N-terminal amino acid blocked
62	ivgrpghqlvmv	61	P10982	Actin-1	75 / 83	15693.88	5.30	Fungi	Cytoskeleton
63	mltelekalnsidsv	68	P05109	Protein S100-A8*	87 / 93	10834.51	6.51	Human	Signal transduction
64	mltelekalnsidsv	68	P05109	Protein S100-A8	87 / 93	10834.51	6.51	Human	Signal transduction
65				N-terminal amino acid blocked
66	lpiheyqsvkllnsy	82	Q5KN95	Succinate-CoA ligase*	87 / 93	44998.54	5.62	Fungi	Energy production and conversion
67				N-terminal amino acid blocked
68	vykvadislaafgrkeiega	104	P39954	Adenosylhomocysteinase*	90 / 95	49125.51	5.83	Fungi	Amino acid transport and metabolism
69	vaywepgtqynygsiveyeg	81	Q8U1H4	Putative chitinase*	65 / 88	39701.99	5.18	Bacteria	Carbohydrate transport and metabolism
70	vaydepgtqynhgsi	54	P47009	Hypothetical 11.7 kDa protein in IDS2-MPI2 intergenic region	64 / 91	11659.54	6.40	Fungi	Unclassified
71				N-terminal amino acid blocked
72				N-terminal amino acid blocked
73	ginkgklg	53	Q5NF19	Probable inorganic polyphosphate /ATP-NAD kinase*	100 / 100	32505.52	8.63	Bacteria	Coenzyme metabolism
74				N-terminal amino acid blocked
75				N-terminal amino acid blocked
76				N-terminal amino acid blocked
77				N-terminal amino acid blocked
78				N-terminal amino acid blocked
79				N-terminal amino acid blocked
80	atavtqgpftevttl	61	Q9Y8B5	Mitochondrial-processing peptidase subunit beta*	60 / 93	51162.66	5.69	Fungi	Amino acid transport and metabolism
81	ateakgaigavktvigavxd	81	Q6FYM3	ATP synthase subunit beta*	60 / 83	57135.98	5.61	Bacteria	Energy production and conversion
82	apegvgdigliglah			No significant hit found
83				N-terminal amino acid blocked
84				N-terminal amino acid blocked
85				N-terminal amino acid blocked
86				N-terminal amino acid blocked
87				N-terminal amino acid blocked
88				N-terminal amino acid blocked
89				N-terminal amino acid blocked
90	papadflkgvdsneeafiqr	66	Q98N59	Elongation factor G*	50 / 77	77208.28	5.28	Bacteria	Translation, ribosomal structure and biogenesis
91	hlqqvf			No signifcant hit found
92	pnqtvfdirdgavyt	62	Q93LL2	Acetyl-coenzyme A synthetase*	62 / 85	37143.22	6.14	Bacteria	Energy production and conversion
93	ahkeikfsnegraai	73	P50142	Heat shock protein 60*	73 / 93	61888.74	5.69	Fungi	Posttranslational modification, protein turnover, chaperones
94	qlgseadftqisfdh			No signifcant hit found
95	lggggvlslg			No signifcant hit found
96				N-terminal amino acid blocked
97				N-terminal amino acid blocked
98	qglpnprqgaed	60	P36627	Cellular nucleic acid-binding protein homolog*	100 / 100	19342.51	8.34	Fungi	Signal transduction
99	rlkvggpggg			No signifcant hit found
100				N-terminal amino acid blocked
101	qtdpaeitidgvfgi	55	P01007	Plasminostreptin*	54 / 85	11398.86	6.07	Bacteria	Defense
102				N-terminal amino acid blocked
103				N-terminal amino acid blocked
104	qglnlpg	50	Q927D8	UPF0133 protein	100 / 100	11438.28	4.59	Bacteria	Unclassified
105				N-terminal amino acid blocked
106	dasnkseygtvigig	60	Q24895	78 kDa glucose-regulated protein precursor*	83 / 92	71675.38	5.09	Insect	Posttranslational modification, protein turnover, chaperones
107	pgqllaqanphgdsg			No signifcant hit found
108	qgqllaqanphg			No signifcant hit found
109				N-terminal amino acid blocked
110				N-terminal amino acid blocked
111	spgnllgdgarg	61	P04922	Circumsporozoite protein precursor	67 / 83	34782.33	5.38	Insect	Unclassified
112	fpapgateqvkgvis			No signifcant hit found
113	aapvstndrfvpsaa	57	P78972	WD repeat containing protein	64 / 91	53417.86	9.30	Fungi	Unclassified
114	aapvslndqfgpsaa	60	Q5A387	Hypothetical Protein MSS1	73 / 91	42895.97	5.98	Fungi	Unclassified
115	gghsvpltnfm	65	P07267	Saccharopepsin precursor*	73 / 100	44499.00	4.70	Fungi	Amino acid transport and metabolism
Asterisks indicate unique proteins identified from S. crispa fruiting body using Tandem MS analysis and Edman sequencing. Commonly identified proteins in S. crispa and H. erinaceum are shown in red letters.